[#item_full_content] For cannabis flower, cannabinoids occur at concentrations far too high for traditional spike recovery experiments to meaningfully assess extraction efficiency. Even large spike additions contribute only a small fraction of the total analyte mass, making recovery results insensitive to extraction performance. This article explains why extraction efficiency for cannabinoid methods must instead be established through matrix-exhaustive, sequential extraction studies. 
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Why Spike Recovery Fails for Cannabis Flower: Using Sequential Extractions to Validate Cannabinoid Extraction Efficiency | Cannabis Science and Technology – Cannabis Industry News, Insights
ByPot Shop News
December 24, 2025